SubtiBank SubtiBank
Version comparison:

2019-02-19 18:41:342025-05-31 11:20:15

locus

BSU36000

BSU_36000

outlinks

bsu

BSU36000

BSU_36000

The protein

Catalyzed reaction/ biological activity

(2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO2 (according to Swiss-Prot)

(2S)-2-acetolactate + H+ --> (R)-acetoin + CO2 (according to UniProt)

The protein

Protein family

alpha-acetolactate decarboxylase family (according to Swiss-Prot)

alpha-acetolactate decarboxylase family (single member, according to UniProt)

Biological materials

Expression vectors

for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP822, available in [SW|Jrg Stlke]'s lab, [pubmed|20389117]

for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP822, available in [SW|Jörg Stülke]'s lab, [pubmed|20389117]

References

19087206, 10986270, 16428414, 16493705, 7685336, 16428414, 20081037, 20389117, 24734205, 22178965, 29809139, 29796971, 23985082, 30039521

19087206, 10986270, 16428414, 16493705, 7685336, 16428414, 20081037, 20389117, 24734205, 22178965, 29809139, 29796971, 23985082, 30039521, 31113899

Gene

Phenotypes of a mutant

reduced acetoin production [pubmed|31113899]

impaired biofilm development [pubmed|31113899]